Journal: BMC Gastroenterology
Article Title: A new model of portal vein thrombosis in rats with cirrhosis induced by partial portal vein ligation plus carbon tetrachloride and intervened with rivaroxaban
doi: 10.1186/s12876-024-03253-4
Figure Lengend Snippet: The establishment of a PVT model in rats with liver cirrhosis and characteristics of extrahepatic macrothrombi and intrahepatic microthrombi. (A) Gross appearance of portal vein thrombosis. As indicated by the arrow, a segment approximately 0.5 cm in length protruded with the black thrombi at the site of ligation of the portal vein (A1, A2). Portal vein HE staining (A3, ×40; A4, ×100). Fibrin immunohistochemical staining (A5, ×40; A6, ×100). (B) Representative images of H&E-stained liver sections (upper panels, ×200) and Sirius red staining (lower panels, ×100) of rat liver tissues at different model groups. (C) The semi-quantitation of Sirius red staining (collagen deposition) in liver, hepatic hydroxyproline content, serum ALT and AST activities of different model groups. The data are expressed as the mean ± SD. N ( n = 7); PPVL ( n = 6); PPVL + CCl 4 4w ( n = 6); PPVL + CCl 4 6w ( n = 5); PPVL + CCl 4 8w ( n = 6); PPVL + CCl 4 10w ( n = 8); * P < 0.05 vs. N, # P < 0.05 vs. PPVL, ## P < 0.01 vs. PPVL
Article Snippet: The hydroxyproline standard (trans-4-hydroxy-L-proline, Cat No. H54409) was purchased from Sigma.
Techniques: Ligation, Staining, Immunohistochemical staining, Quantitation Assay